Kankozha M.K.

The Kazakh National Medical University named after C.D. Asfendiyarov

Republic of Kazakhstan, Almaty

FUNCTION OF ERYTHROCYTIC TRANSPORT OF GLUCOSE

Erythrocytes which are the most numerous part of regular elements of blood have disk-shaped, concave-concave form. Hemoglobin composes the basis of contained erythrocyte. Basic function of erythrocytes not only supply tissues with oxygen and other substances but also removal from them carbonic acid.

According to all available information transport function of erythrocytes is not limited on the surface membranes. Erythrocytes adsorb from plasma glucose, amino acids, proteins, lipids and transfer them to the tissues.

Glucose is basic and most important energy material for maintenance of metabolic processes in the organism of a human and animals. Especially intensively it is consumed by tissues of central nervous system, muscular system etc. More than a half of the energy which expends our organism is formed due to the oxidation.

Consequently, glucose is the most valuable nutrient for the majority of cells which concentration plays leading part in metabolism and determines by balance between its expense and arrival from the food or as a result synthesis in the organism. Maintenance of glucose at the proper level is essential and determining for the vitality.

Disadvantageous action of environment and deficient supply of tissues by oxygen the organism of a human and animals, lead to hypoxia.

Continuous energy consumption is the necessary condition of the vital activity of any biological structure. Hypoxia not only complicates process of disease connected with functional insufficiency of respiratory and cardiovascular systems, diseases of liver, kidneys, endocrine system, etc, but also is defines its outcome.

The manifestations of hypoxia depend on etiology, degrees, speed of development and duration of hypoxic state, reactivity of the organism, in this case occur reduction in the delivery of oxygen to tissues, damage of metabolism and structure of cells, and also adsorptive- transport properties of erythrocytes [1, 2, 3, 4, 5, 6, 7, 8, 9].

The purpose of the present investigation is study of erythrocytic transport of glucose with hypoxia in experiment.

Materials and methods

Experiments were conducted on 64 pedigreeless adult dogs weighing 12-18 kg, which were divided into 2 groups: the 1st group – control group, and for animals of the 2nd group was invented the model of hypoxia.

In experiment blood was separated into plasma and erythrocyte sediment to determine the concentration and content of glucose in blood plasma and on surface of erythrocytes.

Blood was centrifuged for 5 minutes at 1500 rpm for adsorbed substances from the erythrocyte sediment. Thus one part of erythrocytic mass diluted in a volume ratio to three parts of 3% solution of sodium chloride.

The obtained single washing from erythrocytes (suspension) gently mixed and incubated at 37 °C for 5 minutes for the full flush of molecules associated with membrane of erythrocytes. Then erythrocytic suspension was centrifuged at 1500 rpm for 5 minutes. As a result of this procedure in erythrocyte washout passed the substances adsorbed on membrane of red blood cells and supernatant (washing) was transferred into clean tubes [of 10, 11].

Biochemical blood analysis was conducted on the unit “Automatic biochemical analyzer “BioSystems A-25” (Spain)”.

Static processing has been done on the computer. The criteria of Styudent t - was significant at P <0,05.

Results of study and discussion

As a result of studies in the 2nd experimental group of animals on a background of hypoxia, where the number of erythrocytes significantly increased at 18,4%, and hemoglobin level of 9,23% as compared with the control group (Pic. No. 1 and no.2).

Picture No.1 Picture No.2

According to study in blood plasma in the 2nd group in comparison with the control group observed a significant increase of glucose to 6,79+0,09 mmole/liter (control 5,65+0,10 mmole/liter). The percentage of glucose content of glucose in the washings significantly increased by 39,6% compared with the control group.

Sample of rates and adsorptive-transport function of erythrocyte on background of hypoxia, adsorption and transfer of substances by red cells of the blood is the mechanism in response to exposure of unusual factors of environment to organism.

Results of researches indicate that during experimental hypoxia in plasma increases not only red blood but also adsorptive-transport function of erythrocytes.

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