Medicine/8.Morphology
Ulyanov V.O.
The structure of ovaries in posterity of the irradiated rats
Radiation induced damages of genome can be realized
phenotypicaly in the next generations of the irradiated
people and animals. However the state of parenchima
and stroma of ovaries in descendants of irradiated mammals
wasn’t investigated enough. Dependence between the degree of ovary tissue damaging
and amount of pathological changes of gametes and pregnancy process wasn’t
established.
Purpose of work: to study the morphological
changes in ovaries of posterity of the irradiated rats in postnatal ontogenesis.
The rats of experimental
lines Wistar of 3-4 month-old were researched.
Animals were divided into two groups: 1) intact unpregnant
females (n=21); 2) unpregnant females of posterity of the
irradiated rats (n=21).
The males and females
of rats were irradiated by fractional g- irradiation every 72 hours for 0,1 Gy for a
session to achieving a total dose 1,0 Gy. Animals were
coupled after completion of the irradiation. Animals were destroyed from the
experiment on 14, 30 and 90 days of ontogenesis. Ovaries were removed. It was
prepared permanent histological preparations which were painted by hematoxylin-eosin and be van Gysoni.
It was established that in 14-daily females of posterity of the
irradiated rats, the structure of ovaries had expressive enough differences
comparatively with control. The amount of follicles on the plane of section was
more than in control group. The follicles were densely located in a cortical
substance. The cortical substance prevailed over the medullar substance, but
degree of its maturity was smaller. An interstitium was
developed weaker. The follicles were heterogeneous: some parts had a normal kind;
other parts were with the signs of destruction. The nucleuses in a lot of
follicular cells were in the state of karyopicnos and
karyorrhexis. It was set as apoptotic little bodies so mitosis. A theca was
well developed and was presented as densely located light cells, considerably
larger than follicular cells. The ovocytes were without
features, hadn’t differences with the norm. An effect of atresia
was absent. The vessels of the medullar substance were densely located. They
were thin-walled with blood cells inside.
In the 1-month-old posterity of the irradiated rats
the general view of ovaries very looks like the previous term of research (2
weeks). An ovary had a lot of follicles, but the secondary and thirdary follicles prevailed. The interstitium
layers were thicker, than in control group. At supervision above follicles
there was the impression that proliferative processes
left behind the ripening of follicles. The well-shaped follicles with normal ovocytes and well developed transparent and granular
membrane were not enough. The cavities of follicles were undeveloped, often were
absent, without regard to a thick granular area. It is possible to assume that
the secretion of liquid, rich on estrogens, was broken or absent. Follicular
epithelium was with the signs of karyorrhexis, destruction and apoptosis. The
normally formed atretic bodies were absent. There were
large follicles in which a theca was well formed with the differentiated
layers. There were some areas of dilution within the limits of granular layer.
The interstitium was
edematous comparatively with 2 weeks of experience. There were cells with high
functional activity around the follicles in the interstitium.
They were sharply selected between typical cells. They located single. They were
more light and large than circumferential cells. Its cytoplasm was
heterogeneous. The nucleuses were very large, sometimes eccentric with well
expressed chromatin. The structure of these cells was like as glandular cells. The
appearance of these cells with hypertrophic thecacytes
was a compensatory reaction to decreasing of endocrine function of follicle
cells.
The ovaries of 3-mont-old posterity of the irradiated rats looked like
less functionally active organs, comparatively with control. The amount of
ripening follicles on the plane of section was decreased. An effect of atresia was more expressive. At the same time, fully formed
functionally active atretic bodies weren’t enough.
Conclusions. The g-irradiation
of male and female rats before a coupling causes violation of forming of
ovaries tissue in postnatal ontogenesis of their first generation posterity. It
shows up the delay of becoming of connective tissue stroma
and changes of cellular cycle of follicle epithelial cells.