Batezhenko T., prof. Lyashenko V., Bondar O.
Dnipropetrovsk National University named after Oles Gonchar
 
Cation composition of gastric juice and saliva of rats in conditions of prolonged proton pump blocking
 
 

Among digestive diseases the most common are acid diseases. Antisecretory drugs - proton pump inhibitors, which are widely used in the treatment of pathologies of the digestive system, with prolonged use increase the risk of negative aspects associated with morphofunctional and immunological changes of gastric mucosa, due to the electrolyte composition of its secretion.

The aim of our work was to study changes in quantitative and qualitative dynamics of electrolyte composition of gastric juice and saliva of rats on the background of a long block of proton pump, which will let us use the data in the diagnosis of cancer.

The experiment was conducted on white male rats weighing 198-230 g. The rats were divided into 2 groups: the first - control - 36 rats, the second - experimental - 33 rats. Every day the rats of the experimental group were injected intraperitoneally the drug (pharmacological name "Omez" will be used) in 20 mg / kg. At the same time the animals of the control group were injected the appropriate amount of saline. During the month, every 6 days the gastric juice and mixed saliva were collected and investigated in terms of: the mass concentration of potassium, sodium, magnesium and calcium. The content of electrolytes in the investigated sample of gastric juice and saliva was determined on the equipment Capel 103P using capillary electrophoresis system.

The graph shows the contents of K⁺ cations in gastric juice in basal and fasting portions of the studied rats during the experiment.

This figure and the next one along the axis abscess - time observation days, the vertical axis - the number of cations in mg / dm ³. ; Cf – indicators of cations in fasting portion of gastric juice of the control group; KB - performance of cations in basal portion of gastric juice of the control group, Ef – indicators of cations in fasting portion of gastric juice of the experimental group; Eb – figures of cations in basal portion of gastric juice of the experimental group.

The Indicators of cations K⁺ in fasting and basal portions of control group almost unchanged over the experiment. Performance at blocking proton pump in fasting and basal portion of gastric juice significantly increased at 3-4 times, especially at the end of the experiment.

A similar situation was observed when determining the mass concentration of sodium.

In the next figure the mass concentration of Mg cations in the control group practically did not change throughout the experiment. When using PPI the content of cations Mg²⁺ compared with the control group increased and starting from the 24th day rapidly decreased.

Against deficit Mg²⁺ and Ca²⁺ forms a vicious circle. Magnesium-calcium deficiency, causing motor dysfunction of actin filaments and microvilli, leading to further malabsorption of macro-and micronutrients intestinal mucosa, which in turn further enhances motor dysfunction and gastrointestinal mucosal trophism violation.

Such dynamics was observed in the determination of mass concentration of Ca²⁺ cations.

As you can see in the next figure the indicators of the mass concentration of K⁺, Ca²⁺ cations in the saliva of the control group are stable over the time of study. When blocking proton pump performance cations K⁺, Ca²⁺ in saliva significantly decreased at the end of the study.

The opposite situation was observed in the cations Na⁺ Mg²⁺ of mixed saliva. The increase in the number of cations was observed during the experiment in the experimental group.

To sum up, we can make the conclusion that gastric juice may be considered a promising object of research in the study of diseases of the digestive system and their diagnosis.