Kondratyuk R.V., Trivailo M.S., Saverchenko
V.G., shapharenko N.V.
National Technical University
of Ukraine «KPI»
THE Unit for cultivation microorganisms
The
model relates to the microbiological industry, namely, the cultivation of
industrial plants to microorganisms in liquid media and can be used for the
production of biologically active compounds and vaccines.
Known unit
for cultivation microorganisms (UCM), which contains the frame and the platform
rotating in an inclined plane with the vessels for cultivation, and the drive
(see AS USSR 1731801, C 12 M 1 / 10,
1992).
The disadvantage
of known UCM consists in inconvenience of the use, as needs at the use in
packing of working liquid.
The
nearest to the utility model after technical essence and reached effect
accepted for the prototype of UCM, which contains united between itself in
underbodies by a flexible pipeline two cylinder chambers with the technological
union couplings and devices for serving and taking of exhaust sterile air, and
also mechanism of the recurrent-forward moving of chambers in vertical a plane
(see, A.s. USSR ¹1131899, C 12 M 1/00, 1984 fig. 3).
The disadvantage
of known UCM consists in that its chambers have megascopic weight (resource-demanding)
and way of moving, as a result the charges of energy grow on cultivation, and
it results in the increase of prime price of product.
The
basis of the utility model a task to improve UCM, which means change of chambers
is achieved by reducing their weight and the desired movement, which reduces
the cost of energy (the movement of chambers) and leads to lowering the cost of
use.
This
task is solved with UCM, which contains united between itself in an underbody
by a flexible pipeline two chambers with the technological union couplings and
devices for a serve sterile and taking of exhaust air, and also mechanism of
the recurrent-forward moving of chambers, in vertical a plane, in obedience to
an useful model, new is that chambers have a form of spheres.
Marked
a distinctive sign provides, as compared to a prototype, diminishing of weight
and necessary moving of chambers, which decreases expenses energy on their
moving and brings the use over to the decline of cost. Except for it the sizes
of UCM diminish on its height.
Claimed
UCM is schematically represented on a draft, general view.
UCM
contains united between itself a flexible pipeline 1 two chambers 2, 3 with
devices 4, 5 for the serve of sterile air 6 and taking exhaust. Chambers 2, 3
have a spherical form of radius of «R» and added to the mechanism them
irregular recurrent-forward moving in a vertical plane, the occasion of which
is contained by a reversible reducing motor-gear 7 with control block 8 and by
a drum 9, which engulfs thrown over through blocks 10 and added ends to the
chambers rope 11. Cameras 2, 3 have technological connections 12 for a fluid
and tube 13 to connect a flexible pipe 1. Flexible tubing 1 has a valve 14 for
coacervating content cameras and technological ceiling of pipeline.
UCM
works as follows.
One of
the pre sterilized chambers, such as shown in fig. 1 the camera position, fill
to the required level of life-giving liquid H
and planting material (working fluid). Then open the valve 14 in pipeline 1 and
carry out cultivation placed in nutritional liquid cultures. Open the tap 14
pipe 1 leads to the flow of fluid through a pipeline 1 with the camera 2 to
camera 3, displacing it from the air 6. When flow is mixing fluid and increase
its level of H1 in the
chamber 3. After filling the fluid chamber to the desired level 3, control unit
8 switches the motor reducer 7 and change camera position in the vertical
plane: the camera 2 lead in the bottom position, and camera 3 - in the top
position, where they remain for a specified time for transfusion.
Overflow
fluid between the chambers in a different situation occurs in the opposite
direction, that from the camera 3 in the chamber, 2 previously described
method.
After
the required number of cycles of displacement and time transfusion contents
poured into the chamber through valve 14 for further use.
So as
spherical chambers as compared to cylinder in a prototype at the identical
thickness of walls and volume have considerably less weight and need the
diminished way of moving (as a result of less size on a height), charges of
energy on the multicyclic moving of chambers in the process of cultivation
diminish substantially, and it brings the use over to lowering of cost, the
also diminished height of UCM is instrumental in it.