Trivailo M.S., Kondratyuk R.V., shapharenko N.V., Saverchenko V.G.

National Technical University of Ukraine «KPI»

THE SATIATION OF WORKING LIQUID IN BIOREACTORS

An utility model relates to the biotechnology and can be used for cultivation microorganisms in liquid environments at making bioactive matters and vaccines.

Known unit for cultivation microorganisms (UCM), which contains the frame and the platform rotating in an inclined plane with the vessels for cultivation, and the drive (see AS USSR 1731801, C 12 M 1 / 10, 1992).

The disadvantage of known UCM consists in inconvenience of the use, as needs at the use in packing of working liquid.

The nearest to the utility model after technical essence and reached effect accepted for the prototype of UCM, which contains united between itself in underbodies by a flexible pipeline two chambers with the technological union couplings and devices for serving sterile air, and also mechanism of the recurrent-forward moving of chambers in vertical a plane (see, A.s. USSR №1131899, C 12 M 1/00, 1984).

The disadvantage of known UСM consists in relatively to the low productivity as a result of passive satiation of working liquid by air, which is carried out only a diffusive way which diminishes a presence in it of oxygen and slows growth of microorganisms.

In basis of useful model put task of perfection of UCM, in which by modification of connection and location of chambers intensity of satiation of working liquid is provided by air, which promotes maintenance in it air, and consequently, accelerates growth of microorganisms and results in the increase of the productivity.

This task solves in UCM, which contains a frame and two chambers are united with a pipeline with the technological union couplings and devices for the serve of sterile air, and also mechanism of the recurrent-forward moving of chambers in a vertical plane, in obedience to an useful model new is that chambers are united between itself in overhead parts, located at right angles to each other and added to the occasion with possibility of turning (moving) about horizontal axis.

Marked distinctive signs provide at transfusion of working liquid between the chambers passing of it through the air volume which is absent in a prototype, and it promotes the saturation of working liquid air, and consequently, and by oxygen. The increase of oxygen in a working liquid accelerates growth of microorganisms and results in growth of the productivity.

On fig. 1 schematically represented declare UCM, general view; on fig. 2 is an end-view on fig.1; on fig. 3 is position of chambers in the transient state.

UCM contains a fundamental frame (flag) 1 and two, for example, rectangular in a transversal cut, chambers 2, 3 with devices 4 for the serve of sterile air and technological union couplings 5, 6 for taking of exhaust air and priming and coacervating of content of chambers, and also mechanism of the recurrent-forward moving of chambers in a vertical plane as a braking (worm) motor reducing gear 7 with control block 8. Chambers 2, 3 in overhead (under ceiling) parts is connected by between itself a pipeline 9, located at right angles to each other and added to the occasion by a segment fork 10 with possibility of turn about horizontal axis of “O-O” (fig. 2). Fork 10 motionlessly fastened on the initial billow of a 11 motor reducing gear 7, and chambers 2, 3 – fixed from displacements on it, for example, spirally 12 or by known another way. Pipeline 9 contains a faucet 13 for his technological ceiling, and union couplings 6 – faucets 14 the same setting. Reducing motor-gear 7 fastened on a frame 1 screw-bolts 15.

UCM works as follows.

One of preliminary sterilized chambers, for example in rotined on fig. 1 position chamber 2, refuel a nourishing liquid and sowing material (working liquid), whereupon open a faucet 13 on a pipeline 9 and carry out cultivation of the cultures placed in a nourishing liquid.

Opening of faucet 13 results in the crossflow of working liquid on a pipeline 9 from a chamber 2 in a chamber 3, which outpoured from a pipeline in the volume of air 14 splashed and moves together with him. The crossflow of working liquid between chambers causes the decline of its level in a chamber 2 and to the increase of level of H in a chamber 3. After formation of necessary level of H of working liquid in a chamber 3, control block 8 includes a reducing motor-gear 7 and changes position of chambers (fig. 3) in a vertical plane: chamber 2 translates in vertical position, and chamber 3 – in horizontal position in which they remain on set time. The crossflow of working liquid between chambers in the changed position takes place in reverse direction, that from a chamber 3 in a chamber 2.

After a necessity for cultivation of number of moving, content of chambers is united through a faucet 6 for the subsequent use.

So as in declared UCM, unlike a prototype, transfusion of working liquid between chambers is carried out through the volume of air which absents in a prototype, its satiation is increased oxygen of air, and it accelerates growth of microorganisms and results in growth of the productivity.