Karachun V.V., Trivailo M.S., Mel’niсk V. N.

National Technical University of Ukraine «KPI»

THE CULTIVAtION OF CELLS IN THE LIQUID MEDIUMS

The utility model belongs to biotechnology and can be applied for the cultivation of cells in the liquid mediums at manufacturing of biologically-active substances and vaccines.

The known design of the device for the cultivation of cells (DC) which contains vertically placed cylindrical case with the technological branch pipes located in the case on its axis, a hollow shaft with regulated on frequency of the reference by an occasion, the mixer fixed on a shaft in a of the kind four-bar articulate mechanism with shovels, the filter and the aerator (for example, А.c. The USSR №1633814, С12M3/00, 1995).

The lack of this device is the excessive complexity of a design owing to presence at it of the regulated driving and the uneasy form of a mixer which not only raises manufacturing costs, but also reduces reliability of work.

There is also known device which contains vertically mounted cylindrical case with the technological branch pipes, the carving shaft placed in the case with the mixing disk established perpendicularly to its axis and the reversible drive, the guide, and also the aerator (for example, the patent of Ukraine for the utility model, №12121, С12 M3/00,2006).

It is the closest to the utility model by the technical essence and achievable effect and accepted as the nearest analogue.

Its lack consists in complexity of a design owing to presence in it of a carving shaft, the guide and a reversible drive.

The basis of the utility model is a task of improvement DC in which, by change of the arrangement of a shaft and a disk on the shaft their form is optimizing and also the necessity of the equipment of an occasion by the device for change of a direction of movement which serves design simplification is eliminated.

The task is solving by that in the device which contains vertically located cylindrical case with the technological branch pipes, the shaft placed in the case with a mixing disk, the drive and the aerator, according to the offered decision, the new is that the shaft is located under a right angle to a case axis, and the disk is placed along an axis of a shaft and is motionlessly connected to it.

The specified difference, in comparison with the accepted analogue, discharges necessity of use of the mobile carving details, guides, and devices for change of a direction of action of an driving (shaft), that considerably simplifies a design (fig.1).

On the drawing the recommended device is represented schematically.

AC contains vertically located cylindrical case 1 with a branch pipe 2 for introduction of a nutritious liquid and a sowing material (a working liquid), a branch pipe 3 for removal of the cultural liquid, a branch pipe 4 with the aerator 5 and a branch pipe 6 for removal of the fulfilled gas. The shaft 7 with the mixing disk 8 is located in the case 1 under a right angle to its axis. The disk 8 is placed along a wasp of a shaft 7 and motionlessly fixed on it, for example, in its cut by screws 9. The reference of a shaft 7 in a constant direction is carried out by an occasion 10 (for example, a motor-reducer). The case 1 and a disk 8, except cylindrical, can have the rectangular form it (is not shown).

The device works in the following way.

In the preliminary sterilized case, through a branch pipe 2 enters a working liquid 11 (a nutritious liquid and a sowing material) then in the aerator 5 inject gas for aeration of the cultural medium and include a drive 10 owing to what the shaft 7 with a disk 8 comes to rotary movement. Turning around, the disk 8 makes pressure upon a working liquid 11, carrying out its mixing.

As the mass exchange of the working liquid is realized without mechanical moving of a disk on a shaft which in the nearest analogue requires available carving pair, and also guides, and the device for a movement backspacing, so the design of the offered DC essentially becomes simpler.

Except the design simplification, withdrawal of the surfaces contacting with a working liquid of a mechanical friction which take place in the resulted analogues, discharges the damage of cells and pollution by products of a pulling down of a working liquid which assists productivity increase of the cultivation and improves quality of a finished product.