Mel’niсk V. N., Trivailo M.S., Karachun V.V.

National Technical University of Ukraine «KPI»

THE MASS-EXCHANGE BY SYSTEM OF FLAT MIXING SHOVELS

The model concerns to the biotechnology and may be used for the cultivation of cells in liquid mediums at manufacturing of biologically-active substances and vaccines.

The known device for the cultivation of cells (DC) which contains the cylindrical case with technological branch pipes, a hollow shaft with the drive regulated on frequency of the reference, the mixer fixed on a shaft in a form of the four-bar articulate mechanism with shovels, the filter and an aerator (А.c. The USSR № 1633814, С12 M3/00, 1995).

Lacks of this DC consist in complexity of installation owing to presence of the regulated drive and the difficult form of a mixer, in non-uniform mixing of a working liquid on its height and diameter, pollution of a working liquid by products of a pulling down which result from a mechanical friction in the articulate mechanism and the damage of cells.

There is also known DC which contains vertically located cylindrical case with the technological branch pipes, two shaft established one in enother placed in the case on its axis with radially attached to them mixing shovels, a drive and an aerator (А.c. The USSR № 1730135, С12 M3/00, 1992).

This DC is the closest to the utility model for a technical essence and achievable effect and accepted as a prototype.

The known DC is more simple than previous, but it also has a mixing lack (mass-exchange) of a working liquid in a direction of diameter (i.e. the central and peripheral parts) and in a direction of height owing to a coaxial arrangement of shaft and lateral joining to them of the mixing plates which slows down growth of cells, and so, reduces productivity, and is its basic lack.

Besides, the known DC has a difficult design and raised material capacity which serve as its other lacks.

The basis of the model is a task of improvement of the DC by change by an arrangement of shaft and joining to them the mixing shovels. Under these conditions increase of intensity of mixing of a working liquid in the case on its diameter and height which leads to productivity growth at simultaneous simplification of a design is provided and decrease of the material capacity.

The task is solving by that in DC which contains vertically located cylindrical case with the technological branch pipes, two shaft with the mixing shovels placed in the case along its axis, the drive and the aerator, according to the utility model the new is that shaft are placed opposite (i.e. not coaxial) concerning a case axis, and a shovel joining to their end faces.

The specified difference, in comparison with a prototype, allows to intensify mixing of external (peripheral) and central parts of a biomass and to raise uniformity of mixing on height which accelerates growth of cells and leads to productivity growth. Besides, the length of the shaft and number of the mixing shovels and cogwheels in an occasion which simplifies a design and it’s material capacity is several times reduced.

The essence of the utility model is explain by the drawing on which the offered DC is schematically represented, a longitudinal section.

DC contains vertically located cylindrical case 1 with a branch pipe 2 for introduction of a nutritious liquid and a sowing material, a branch pipe 3 for removal of a finished product, a branch pipe 4 for removal of the fulfilled gas and a branch pipe 5 with the aerator 6. In the case 1, identical shafts 7, 8 located opposite concerning to its axis, to which end faces attached the mixing shovels 9. Shafts 7, 8 are attached to a motor-reducer 10 established on the cover 11 of the case 1.

DC works in the following way.

In the preliminary sterilized DC case 1 through a branch pipe 2 enter a nutritious liquid and a sowing material (subculture) then in aerator 6 inject gas for aeration of the cultural medium and turn on the motor-reducer 10 which gives the reference to shafts 7, 8 with shovels 9. Turning around, shovels 7, 8 put to a working liquid 12 difficult movements and mix it.

Because the shafts 7, 8 are placed opposite concerning an axis of the case 1, instead of a coaxial arrangement in a prototype, shovels 9, except granting of circular movement round an axis of the case 1, one part grasp the central (internal) layers 13 working liquids

12 and move them radially to an external (peripheral) part of 14 cases, and other part on the contrary, to other party.

Owing to movement of a working liquid in a radial direction, there is also its additional mixing which improves ability to live of cells and leads to productivity growth. And with it, design of DC becomes simpler and decreases the material capacity owing to reduction of the sizes of shafts and number of shovels and cogwheels in an the drive.`