Karachun V.V., Trivailo M.S., Mel’niсk V. N.

National Technical University of Ukraine «KPI»

THE ACTIVE MIXING PERIFERAL LAYERS WORKING LIQUID

The model concerns to the biotechnology and can be used for the cultivation of cells in liquid mediums at manufacturing of vaccines and biologically-active substances.

The known device for the cultivation of cells (DC) which contains the cylindrical case with technological branch pipes, a hollow shaft with the drive regulated on frequency of the reference, the mixer fixed on a shaft in a kind of the four-bar articulate mechanism with mixing elements, the filter and the aerator (А.c. The USSR № 1633814, С12 M3/00, 1995).

Lacks of this DC consist in complexity of a design owing to presence in it of the difficult form of the regulated drive and a mixer and pollution of a working liquid by products of a pulling down which arise owing to a mechanical friction in the articulate mechanism.

There is also known DC which contains vertically located cylindrical case with the technological branch pipes, a shaft placed in the case on its axis with radially attached to it mixing elements in the form of shovels, a drive and an aerator (А.с. The USSR № 1555353, C12 M 1/04, 1990).

This DC is the closest to the model for a technical essence and achievable effect and are accepted as a prototype.

The known DC is more simple than previous, but it in the mixing does not provide active mixing among benthonic with top and central with peripheral layers, a working liquid which reduces efficiency of mixing (mass exchange) and it is its basic lack.

Absence of the active mixing of the specified layers slows down growth of cells owing to non-uniform distribution of nutrients (and cells) in volume of a working liquid which leads to decline of productivity.

The basis of the model is a task of improvement DC by change of the form and an arrangement of the mixing elements. Under these conditions mixing among benthonic with top and central with peripheral layers of a working liquid which accelerates growth of cells is intensified and leads to productivity growth.

The given task is solving by that in DC which contains vertically located cylindrical case with the technological branch pipes, the shaft placed in the case with the mixing elements, the drive and the aerator, according to the utility model the new is that mixing elements are executed in the form of cylindrical tubes which are located aslant to a shaft axis.

The specified distinctive sign, in comparison with a prototype, allows to intensify mixing among benthonic with top and central with peripheral layers of a working liquid different in density which raises uniformity of mixing of a working liquid on its volume, and it accelerates growth of cells and leads to productivity growth.

The essence of the model is explain by fig. 1 on which the offered DC is schematically represented, a longitudinal section.

DC contains cylindrical case 1 located vertically with a branch pipe 2 for introduction of a nutritious liquid and a sowing material, a branch pipe 3 for removal of the finished product, a branch pipe 4 for removal of the fulfilled gas and a branch pipe 5 with the aerator 6. The shaft 7 is located in the case 1 along its axis with the drive in the form of a motor-reducer 8 and the mixing elements 10 radially attached, for example, with the help of the rods 9. The mixing elements 10 are made of he tubes with length «l» and internal diameter «d», which are aslant, under a corner «α», attached to the shaft axis 7 and are completely submerged in the working liquid 11.

DC work in the following way.

To the primarily sterilized case 1 through the branch pipe 2 input the nutritious liquid and the sowing material (subculture), after that in the separator 6 through the branch pipe 5 feed the gas for the aeration of the cultural medium and turn on the motor-reducer 8, which rotates the shaft 7 with the mixing elements 10. The mixing elements 10 make a pressure on the working liquid 11 and mix it. Under these conditions, the centrifugal forces with the resultants 12 arise in the working liquid due to the gyration, which projections 13 cause the moving of the working liquid on the futilities of the mixing elements from its natural and centrals layers to the surface and periphery, as it is shown by the arrows at the draft. As like as the pump the pipes pump across their cavity the working liquid. The flow of the working liquid across the cavities of the mixing elements (pipes) 10, which is absent in the prototype, cause its additional mixing, which improve the vital functions of cells and leads to the productivity growth. Except this, the power waste on the mixing decrease, as the cylindrical form of the mixing facilities comparing with the flat form in the prototype, provide the essential decrease the drag during the movement of the mixing elements in the working liquid under other conditions. (И. Е. Идельчик, Справочник по гидравлическим сопротивлениям, М., Машиностроение, 1975, с. 402-403).

After the end of the process of the cultivation, stop the drive 8, and the finished product merge through the branch pipe 3 for the following use.