Karachun V.V., Trivailo M.S., Mel’niсk V. N.

National Technical University of Ukraine «KPI»

THE intensification of transference of culture broth

The utility model refers to microbiology and may be used for cultivation of microorganisms in liquid environments at producing biologically-active substances and vaccines.

It is known the device for cultivation of microorganisms (DCM) which contains a inclined installed on the frame with opportunity to turn round its axis platform with pins (fingers, rods) on its butt end, located with air gap (for shaking) between pins cylindrical in their cross-cut volume for placing of working liquid and a drive (for example, AC of USSR № 1731801, С12М1/10, 1992)

The fault of this DCM consists in considerable level of vibration and noise that appear in consequence of presence of air gaps between pins of platform and volumes.

It is known the DCM which contains horizontal installed on the frame with the possibility of turning around in vertical plate drum with longitudinal sections and placed in sections of drum parallel to its axis cylindrical in cross-cut volumes for placing of working liquid, and also a drive (for example AC of USSR № 499293, С12В1/10, 1976).

This DCM is the nearest to the proposed engineering solution because of its technical essence and reaching effect and is accepted as the nearest analogue.

The fault of the known DCM consists in low effectiveness of mixing of working liquid by the length of volumes, and so by their volume, that slows down the growth of microorganisms and leads to the decrease of productivity.

The mentioned fault is conditioned of that the volumes for placing of working liquid have circular cylindrical shape in their cross-cut, and that is why they do not ensure the change of shape (and sizes) of volume of working liquid while turning around.

In the basis of the utility model it is putted the problem of improvement of DCM, in which by means of introduction in the construction each of the volumes of extra element ensures by the growth of transference of working liquid by its volume, especially by the length of volumes, that intensifies the mass-exchange in biomass and leads to the growth of productivity.

The putted problem is solved by that in the DCM which contains horizontal installed on the frame with the possibility of turning around in vertical plate drum with longitudinal sections and placed in sections of drum volumes for placing of working liquid, and also a drive, according to proposed utility model the new is that each volume for placing of working liquid is equipped with joined to the bottom and side walls longitudinal dividing wall, which is situated with air gap relative to opposed to the bottom walls of volume and is placed in its diametrical plate.

The equipping of each volume with joined to the bottom and side walls longitudinal dividing wall, which is situated with air gap relative to opposed to the bottom walls of volume and is placed in its diametrical plate, ensures the overflowing of working liquid along the volumes at turning (with simultaneous change of its height), that provokes its extra mixing, and that activates the growth of microorganisms and leads to the growth of productivity.

On the fig. 1, а it is schematically presented the proposed DCM, on the fig. 1, b –crosscut A-A on the fig. 1, а; on fig. 1, c – the change of the shape of volume of working liquid in case of turning of drum at the angle 90°.

The DCM contains horizontal installed on the frame 1 with the possibility to turn around its axis drum 2 with sections 3, 4, in which there are situated volumes 5 for placing of working liquid 6. Drum 2 is carried out in a form of opened at one end box 7 with longitudinal dividing wall 8 and joined by bottom to the turning of drive 9 and shaft 10.bottom 11 and side walls 12 with transversal dividing wall 13, which is placed with air gap H relative to opposite to the bottom walls 14 volumes and is situated in its diametrical plate. Against the axial displacement in sections 3, 4 of drum 2 the volumes 5 era fixated by elements of fixation 15.

Accept of determined on drawing, drum 2 can have another known shape and a couple of dividing walls 8, and also inclined location (not shown).

The DCM works in next way.

After filling sterilized volumes 5 with working liquid 6 (nutrient liquid and sowing material) they are locating in section 3, 4 of drum 2, fasting with fixing elements 15 against removals and turn on the drive 9, consequently the drum 2 and the volumes 3, 4 comes into rotary movement. At the turning it happens the transfusion of working liquid along the wallsides of volumes 3, 4, that causes, as in the nearest analogue, its mixing. Simultaneous with the transfusion, it happens the overflowing of working liquid because of created by dividing wall 13 air gap H from one part (compartment) of volume 5 into another, filled up with air, part. Thus, for example, under the turning of drum 2 from the initial (fig. 1, b) position on the angle 90° (fig. 1. c), almost half a working liquid 6, that locates under the dividing wall 13 overflows to another her part, from compartment 16 into compartment 17, and at another turning of drum at the angle 90° (not shown) – working liquid will completely fill up the compartment 17. The overflowing of working liquid 6 to different sides of dividing wall 13, in contrast to the nearest analogue, provokes extra mixing of along the length of volumes 5, owing to what the intensity of mixing as liquid by itself as contacting with it gas (air) grows up.

The growth of intensity of mixing (mass-exchange) leads to growth of microorganisms that lead to the growth of productivity.